pH Tanges of Common Buffers Used in HPLC - HPLC Primer
Date: 14-APRIl-2020 Last Updated: 1-DECEMBER-2025
The table below shows a list of common buffers and their working ranges for HPLC:
The table below shows a list of common buffers and their working ranges for HPLC:
| Buffer | pH Range |
| Trifluoroacetic acid TFA | 1.5-2.5 |
| Phosphoric acid/monobasic phosphate (pKa 1) | 1.1-3.1 |
| Formic acid/formate | 2.8-4.8 |
| Acetic acid/acetate | 3.8-5.8 |
| Mono/dibasic phosphate (pKa 2) | 6.2-8.2 |
| Ammonia | 8.2-10.2 |
| 1-methylpiperidine | 9.1-11.1 |
| Triethylamine TEA | 10.0- 12.0 |