Hydrocortisone Assay Alternate L1 Column – AppNote
May 24, 2021
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Alternate L1 column used for a similar USP assay method of Hydrocortisone
This Similar USP Hydrocortisone Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.5%.
This demonstrates a good alternate Column for your Hydrocortisone USP Method.
Column: Cogent MicroBee C18™, 5 um, 125 Å
Catalog No.: 59305-03
Dimensions: 3.9 x 300 mm
Mobile Phase: 25:75 Acetonitrile / DI Water
Injection vol.: 15 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Sample Preparation: Hydrocortisone 50 μg / mL in Methanol
Note 1: Hydrocortisone, a Corticosteroid, and is similar to a natural hormone produced by your Adrenal Glands. Hydrocortisone is used to treat immune, inflammatory, and neoplastic conditions.
Note 2: The Cogent MicroBee C18™ performs very similarly to the Waters® µBondapak C18®.
This Similar USP Hydrocortisone Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.5%.
This demonstrates a good alternate Column for your Hydrocortisone USP Method.
Peak:
Hydrocortisone
Column: Cogent MicroBee C18™, 5 um, 125 Å
Catalog No.: 59305-03
Dimensions: 3.9 x 300 mm
Mobile Phase: 25:75 Acetonitrile / DI Water
Injection vol.: 15 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Sample Preparation: Hydrocortisone 50 μg / mL in Methanol
Note 1: Hydrocortisone, a Corticosteroid, and is similar to a natural hormone produced by your Adrenal Glands. Hydrocortisone is used to treat immune, inflammatory, and neoplastic conditions.
Note 2: The Cogent MicroBee C18™ performs very similarly to the Waters® µBondapak C18®.