Ivermectin Analyzed with HPLC - AppNote
February 28, 2022
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A Robust Method for Analysis of an Antiparasitic
Medication
A robust and reproducible Method has been developed for this anthelmintic agent. The data below (an overlay of 10 chromatograms ) illustrates both excellent Peak Shape and Retention using this easy Method.
Column: Cogent Phenyl Hydride™, 4 μm, 100 Å
Catalog No.: 69020-15P
Dimensions: 4.6 x 150 mm
Mobile Phase:
-- A: DI Water with 0.1% Formic Acid
-- B: Acetonitrile with 0.1% Formic Acid
Gradient
Injection vol.: 5 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Diluent: 50:50 DI Water / Acetonitrile
Sample Preparation: Ivermectin prepared as 6 μg / mL Standard Solution in Diluent.
Notes : Ivermectin is a semi-synthetic Antiparasitic Medication derived from Avermectins, a class of highly-active broad-spectrum Antiparasitic Agents isolated from the fermentation products of Streptomyces avermitilis. This drug is used in the treatment of Onchocerciasis, but may also be effective against other worm infestations (such as Strongyloidiasis, Ascariasis, Trichuriasis and Enterobiasis). Applied topically, it may be used in the treatment of head lice infestation.
Notes : Calculation for Capacity Factor - Relative Retention k = (tR-t0)/t0
A robust and reproducible Method has been developed for this anthelmintic agent. The data below (an overlay of 10 chromatograms ) illustrates both excellent Peak Shape and Retention using this easy Method.
Peak
1. Ivermectin
Column: Cogent Phenyl Hydride™, 4 μm, 100 Å
Catalog No.: 69020-15P
Dimensions: 4.6 x 150 mm
Mobile Phase:
-- A: DI Water with 0.1% Formic Acid
-- B: Acetonitrile with 0.1% Formic Acid
Gradient
| Time (minutes) | %B |
|---|---|
| 0 | 30 |
| 1 | 30 |
| 5 | 75 |
| 6 | 75 |
| 7 | 30 |
| 10 | 30 |
Injection vol.: 5 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Diluent: 50:50 DI Water / Acetonitrile
Sample Preparation: Ivermectin prepared as 6 μg / mL Standard Solution in Diluent.
t
0
: 1.6 Minutes
K: 4.53
%RSD of 10 injections: ‹0.1%
K: 4.53
%RSD of 10 injections: ‹0.1%
Notes : Ivermectin is a semi-synthetic Antiparasitic Medication derived from Avermectins, a class of highly-active broad-spectrum Antiparasitic Agents isolated from the fermentation products of Streptomyces avermitilis. This drug is used in the treatment of Onchocerciasis, but may also be effective against other worm infestations (such as Strongyloidiasis, Ascariasis, Trichuriasis and Enterobiasis). Applied topically, it may be used in the treatment of head lice infestation.
Notes : Calculation for Capacity Factor - Relative Retention k = (tR-t0)/t0