Calculation of Extra-Column Volume
Detector Absorbance Linearity 100% - 0.1%
Extra Column Volume
Gradient Dwell Volume
Gradient Qualification Tests
Injector Percent Carry-Over
Primary Test Chromatogram
Sample Template Results
Short Term Dynamic Noise
Spectrum of Caffeine in RTM
Wavelength Accuracy from
Wavelength Accuracy – Primary Standard
Spectrum of NIST SRM 2034 (lot95) using an HP 8453 spectrophotometer (bandwidth 1nm). Official values are rounded to nearest nanometer. The bands with a * are recommended for use as calibration bands for HPLC and or CE detectors.
Effect of Extra Column Volume on Your Results
Below are two chromatogram examples of how two different instruments can produce very different results using the exact same conditions. They were both tested, using the exact same certified, HPLC Column, with the exact same NIST traceable standards on the exact same day. These differing results of an older and newer HPLC instrument may be simply they are plumbed quite differently.
Recommendation: Make sure all fittings are new, secure and that all tubing is well cut and as short as possible. Not all HPLC instruments offer optimal extra volume; check with your instrument manufacturer on how to optimize your system.
Hint! Use the MicroSolv™ PQ Kit™ for determining system, extra column volume before transferring methods to different instruments, departments or clients.