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Autosampler Precison

Autosampler Precison
 


 

Calculation of Extra-Column Volume

Calculation of Extra-Column Volume
 


 

Detector Absorbance Linearity 100% - 0.1%

Detector Absorbance Linearity 100% - 0.1%
 


 

Extra Column Volume

Extra Column Volume
 


 

Gradient Dwell Volume

Gradient Dwell Volume
 

 



 

Gradient Qualification Tests

Gradient Qualification Tests
 

 



 

Injector Percent Carry-Over

Injector Percent Carry-Over
 

 



 

Primary Test Chromatogram

Primary Test Chromatogram
 

 



 

Sample Template Results

Sample Template Results
 


 

Short Term Dynamic Noise

Short Term Dynamic Noise
 

 



 

Spectrum of Caffeine in RTM

Spectrum of Caffeine in RTM
 

 



 

Wavelength Accuracy from
Calibration Standard

Wavelength Accuracy from Calibration Standard
 

 

 

Wavelength Accuracy – Primary Standard

Wavelength Accuracy – Primary Standard


 

Spectrum of NIST SRM 2034 (lot95) using an HP 8453 spectrophotometer (bandwidth 1nm). Official values are rounded to nearest nanometer. The bands with a * are recommended for use as calibration bands for HPLC and or CE detectors.


 



Effect of Extra Column Volume on Your Results

Below are two chromatogram examples of how two different instruments can produce very different results using the exact same conditions. They were both tested, using the exact same certified, HPLC Column, with the exact same NIST traceable standards on the exact same day. These differing results of an older and newer HPLC instrument may be simply they are plumbed quite differently.
 

Effect of Extra Column Volume on Results


Recommendation
: Make sure all fittings are new, secure and that all tubing is well cut and as short as possible. Not all HPLC instruments offer optimal extra volume; check with your instrument manufacturer on how to optimize your system.

Hint! Use the MicroSolv™ PQ Kit™ for determining system, extra column volume before transferring methods to different instruments, departments or clients.

 

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