HPLC Column, Amide, 4um, 4.6mm ID x 50mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑05P Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase provides strong, consistent interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface improves retention stability compared to conventional bonded‑silica amide phases.
This column is optimized for laboratories seeking reliable HILIC performance with short run times on standard analytical HPLC systems.
Application Focus: Amide Chemistry for HILIC Separations
Amide stationary phases are well suited for the analysis of highly polar compounds that require stable HILIC retention. The Amide phase is commonly selected for:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds
The TYPE‑C™ silica hydride backbone supports consistent retention and improved reproducibility, reducing variability associated with moisture‑sensitive silica surfaces.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to traditional amide HILIC columns, this column offers:
Faster equilibration between injections
Improved retention stability over variable mobile‑phase compositions
Robust column lifetime under high‑organic HILIC conditions
Reduced water‑layer variability, improving reproducibility
These benefits make the Amide TYPE‑C™ column suitable for both routine analysis and method development.
Why Choose the 4.6 mm × 50 mm Format
The 4.6 mm ID × 50 mm length configuration is designed for fast analytical separations with good resolution:
Short run times with efficient separation
Standard analytical flow rates compatible with most HPLC systems
Good resolving power for polar HILIC analytes
Ideal for high‑throughput analytical workflows
This format is frequently selected when speed and robustness are prioritized over maximum plate count.
Particle Size and Performance
The 4 µm particle size provides a practical balance between efficiency and pressure drop, delivering consistent peak shape and reliable performance on standard analytical HPLC systems.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.