HPLC Column, Silica-C, 4um, 4.6mm ID x 50mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑05P Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing distinct selectivity and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, resulting in stable retention behavior and rapid equilibration—especially advantageous in normal‑phase chromatography.
This column is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica columns often present moisture sensitivity and longer equilibration times.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for compounds that are not soluble in water or require non‑polar solvents. The Silica‑C™ phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvent systems or HILIC conditions
The silica hydride surface delivers reproducible retention and reliable selectivity, outperforming conventional silica in moisture‑sensitive methods.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Unique selectivity, particularly beneficial for normal‑phase workflows
These attributes make Silica‑C™ a dependable solution for consistent, high‑throughput analytical separations
Why Choose the 4.6 mm × 50 mm Format
The 4.6 mm ID × 50 mm length configuration is designed for fast analytical separations and high‑throughput screening:
Short run times with good resolution
Rapid equilibration supporting high sample throughput
Standard analytical flow rates compatible with most HPLC systems
Excellent robustness for routine screening and QC workflows
This size is a very popular choice for laboratories running high‑pressure, high‑throughput screening methods where speed and consistency are essential.
Particle Size and Performance
The 4 µm particle size provides reliable efficiency with manageable backpressure, delivering consistent peak shape and reproducibility on standard analytical HPLC systems while supporting high‑throughput operation.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.
The Silica-C™ HPLC Columns are made with Cogent TYPE-C™ Silica,