Catalog Number - 40000-05P-2

The 40000‑05P‑2 Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing unique selectivity and robust performance for HILIC and normal‑phase separations. Unlike bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, delivering stable retention and rapid equilibration—particularly advantageous in normal‑phase workflows.

This column is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where conventional silica can suffer from moisture sensitivity and longer equilibration times.

Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations

Silica‑C™ stationary phases are selected for the analysis of compounds that are poorly soluble in water or require non‑polar solvents. The Silica‑C™ phase is particularly effective for:

• Lipids

• Peptides and proteins

• Oligonucleotides

• Other analytes requiring non‑polar solvents or normal‑phase conditions

The silica hydride surface provides reproducible retention and improved method stability relative to traditional silica phases.

Key Advantages of Silica‑C™ TYPE‑C™ Technology

Compared to conventional bonded‑silica columns, Silica‑C™ columns offer:

• Faster equilibration between injections

• No need to control moisture in mobile‑phase solvents

• Robust column lifetime under demanding solvent systems

• Distinct selectivity, especially beneficial in normal‑phase separations

These attributes make Silica‑C™ a reliable choice for consistent, non‑aqueous analytical workflows.

Why Choose the 2.1 mm × 50 mm Format

The 2.1 mm ID × 50 mm length configuration is optimized for speed, efficiency, and LC‑MS compatibility:

• Short run times and high throughput

• Rapid equilibration between injections

• Reduced solvent consumption

• Lower flow to the detector or MS source, improving sensitivity

This short, narrow‑bore format is a popular choice for LC‑MS methods when fast analysis is required.

Particle Size and Performance

The 4 µm particle size provides reliable efficiency with manageable backpressure, supporting robust performance on standard analytical HPLC systems while maintaining good peak shape and reproducibility.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.