CUSTOM ITEM: HPLC Column, Silica-C, 4um, 3.0mm ID x 50mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑05P‑3 Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing distinct selectivity and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, delivering stable retention behavior and rapid equilibration—particularly advantageous in normal‑phase workflows.
This column is produced as a custom, made‑to‑order item and is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica columns may suffer from moisture sensitivity and longer equilibration times.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are poorly soluble in water or require non‑polar solvents. The Silica‑C™ phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvents or normal‑phase conditions
The silica hydride surface provides reproducible retention and improved method stability relative to traditional silica phases.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to conventional bonded‑silica columns, Silica‑C™ columns offer:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Distinct selectivity, especially beneficial in normal‑phase separations
These attributes make Silica‑C™ a reliable choice for consistent, non‑aqueous analytical workflows.
Why Choose the 3.0 mm × 50 mm Format
The 3.0 mm ID × 50 mm length configuration provides performance comparable to traditional 4.6 mm ID analytical columns, while offering meaningful efficiency advantages:
Similar run times to 4.6 mm ID columns
Reduced mobile‑phase consumption
Lower operating flow rates
Lower backpressure, supporting broader system compatibility
This format allows laboratories to save solvent and operating cost without requiring special low‑flow or UHPLC‑only equipment.
Particle Size and Performance
The 4 µm particle size delivers reliable efficiency with manageable backpressure, supporting robust performance on standard analytical HPLC systems while maintaining good peak shape and reproducibility.
System Requirements and Flow Rates
Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable
Please contact Customer Service for formal quotation, lead time, and ordering assistance. More details are available under Custom Orders.