HPLC Column, Silica-C, 4um, 4.6mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑10P Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing distinct selectivity and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, resulting in stable retention behavior and rapid equilibration—particularly advantageous in moisture‑sensitive normal‑phase workflows.
This column is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica can present variability due to water adsorption.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar solvent systems. The Silica‑C™ phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvents or HILIC conditions
The silica hydride surface delivers reproducible retention and reliable selectivity, outperforming conventional silica in applications where moisture control is problematic.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Unique selectivity, especially beneficial in normal‑phase separations
These attributes make Silica‑C™ a dependable solution for consistent, high‑throughput analytical separations.
Why Choose the 4.6 mm × 100 mm Format
The 4.6 mm ID × 100 mm length configuration is often selected when additional resolution is required but 50 mm columns do not provide sufficient separation, while still maintaining efficient analytical throughput:
Greater resolving power than 50 mm formats
Shorter run times than longer analytical columns
Standard analytical flow rates compatible with most HPLC systems
Excellent robustness for routine analysis and screening workflows
This format offers an effective balance between resolution, speed, and system compatibility.
Particle Size and Performance
The 4 µm particle size delivers reliable efficiency with manageable backpressure, supporting consistent peak shape and reproducibility on standard analytical HPLC systems while providing improved resolution over shorter column lengths.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.