HPLC Column, Silica-C, 4um, 2.1mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑15P‑2 Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing distinct selectivity, rapid equilibration, and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, delivering stable retention and improved method reproducibility—especially advantageous in moisture‑sensitive normal‑phase workflows.
This column is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica columns may present variability due to water adsorption.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar solvent systems. The Silica‑C™ phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvents or HILIC conditions
The silica hydride surface supports reproducible retention and improved method stability compared to conventional silica phases.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Distinct selectivity, particularly beneficial in normal‑phase separations
These attributes make Silica‑C™ a dependable solution for consistent, non‑aqueous analytical workflows.
Why Choose the 2.1 mm × 150 mm Format
The 2.1 mm ID × 150 mm length configuration is commonly selected when expanded resolution and sensitivity are required, particularly for LC‑MS applications:
Higher resolving power than 50 mm and 100 mm formats
Increased peak capacity for complex mixtures
Reduced solvent consumption compared to larger‑diameter columns
Excellent LC‑MS compatibility, with lower solvent flow to the MS source
This narrow‑bore, long‑column format is ideal when separation confidence takes priority over the shortest run time.
Particle Size and Performance
The 4 µm particle size provides reliable efficiency with manageable backpressure, delivering consistent peak shape and reproducibility on standard analytical HPLC systems while enabling high‑resolution separations of closely eluting analytes.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery
Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.