HPLC Column, Silica-C 300, 5um, 4.6mm ID x 150mm Length, 300A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑15P‑3M Silica‑C™ 300 HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering distinct selectivity, rapid equilibration, and robust performance for HILIC and normal‑phase separations of large molecules. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, resulting in stable retention behavior and excellent reproducibility—especially important in moisture‑sensitive normal‑phase workflows.
With a 300 Å pore size and 5 µm particle diameter, this column is optimized for the separation of macromolecules that require increased pore accessibility.
Application Focus: Silica‑C™ 300 for Large‑Molecule HILIC & Normal‑Phase Separations
Silica‑C™ 300 stationary phases are selected for analyzing large compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for:
Proteins
Large peptides
Oligonucleotides
Polysaccharides
Other macromolecules requiring non‑polar solvents or HILIC conditions
The silica hydride surface provides moisture‑tolerant performance and reproducible retention, outperforming conventional silica in large‑molecule normal‑phase applications.
Key Advantages of Silica‑C™ 300 TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ 300 columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Improved access to large analytes due to wide pore structure
These attributes make Silica‑C™ 300 a dependable solution for consistent, large‑molecule analytical separations.
Why Choose the 4.6 mm × 150 mm, 300 Å Format
The 4.6 mm ID × 150 mm length with 300 Å pores is a widely used analytical configuration for macromolecule separations:
High resolving power for large, complex analytes
Standard analytical flow rates compatible with most HPLC systems
Excellent suitability for UV detection
LC‑MS compatible when used with an appropriate flow splitter
This format is commonly selected when expanded pore access and resolution are required for large molecules.
Particle Size and Performance
The 5 µm particle size provides a practical balance of resolving power, permeability, and robustness, supporting stable separations of macromolecules with manageable backpressure on standard analytical HPLC systems.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
LC‑MS note: Use of a flow splitter is recommended when coupling to mass spectrometry
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.
The Silica-C™ HPLC Columns are made with Cogent TYPE-C™ Silica,