HPLC Column, Silica-C, 4um, 4.6mm ID x 250mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑25P Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing distinct selectivity, rapid equilibration, and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, resulting in stable retention behavior and excellent reproducibility—particularly advantageous in moisture‑sensitive normal‑phase workflows.
This column is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica columns may present variability due to water adsorption.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar solvent systems. The Silica‑C™ phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvents or HILIC conditions
The silica hydride surface supports reproducible retention and improved method stability compared to conventional silica phases.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Distinct selectivity, especially beneficial in normal‑phase separations
These attributes make Silica‑C™ a dependable solution for consistent, non‑aqueous analytical workflows.
Why Choose the 4.6 mm × 250 mm Format
The 4.6 mm ID × 250 mm length configuration is selected when maximum separation and extended retention are required:
Highest resolving power within the 4.6 mm analytical family
Extended retention for closely related compounds
Excellent peak capacity for complex mixtures
Standard analytical flow rates compatible with most HPLC systems
This long‑column format is commonly chosen when separation confidence and resolution take priority over shortest run time.
Particle Size and Performance
The 4 µm particle size delivers reliable efficiency with manageable backpressure, maintaining consistent peak shape and reproducibility on standard analytical HPLC systems while enabling maximum‑resolution separations over extended column lengths.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.