CUSTOM ITEM: HPLC Column, Silica-C, 4um, 3.0mm ID x 250mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑25P‑3 Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering distinct selectivity, rapid equilibration, and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, resulting in stable retention behavior and excellent reproducibility—particularly advantageous in moisture‑sensitive normal‑phase workflows.
This column is produced as a custom, made‑to‑order item and is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica columns may present variability due to water adsorption.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar solvent systems. The Silica‑C™ phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvents or HILIC conditions
The silica hydride surface supports reproducible retention and improved method stability compared to conventional silica phases.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Distinct selectivity, particularly beneficial in normal‑phase separations
These attributes make Silica‑C™ a dependable solution for consistent, non‑aqueous analytical workflows.
Why Choose the 3.0 mm × 250 mm Format
The 3.0 mm ID × 250 mm length configuration is selected when maximum resolution and extended retention are required, while maintaining solvent efficiency:
Superior resolving power and peak capacity for closely related compounds
Comparable run times to 4.6 mm ID columns
Significantly reduced solvent consumption versus larger‑diameter formats
Lower operating flow rates and backpressure, improving system compatibility
This solvent‑saving format is commonly chosen for LC‑MS methods and demanding analytical separations where separation confidence is paramount.
Particle Size and Performance
The 4 µm particle size provides reliable efficiency with manageable backpressure, supporting robust performance on standard analytical HPLC systems while enabling maximum‑resolution separations over extended column lengths.
System Requirements and Flow Rates
Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable