Prep HPLC Column, Silica-C, 4um, 100A. 21.2mm ID x 100mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P21‑100 Silica‑C™ Preparative HPLC Column is engineered for preparative purification and compound isolation when higher loading capacity and improved separation are required beyond semi‑preparative formats. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers efficient recovery, fast equilibration, and robust performance for preparative HILIC and normal‑phase workflows.
The silica hydride surface supports moisture‑tolerant operation and stable retention behavior, enabling reliable yields and consistent turnaround in preparative‑scale separations.
Preparative Use Case: Why 21.2 mm ID × 100 mm
The 21.2 mm internal diameter classifies this column as full preparative, supporting high sample loading and large fraction collection volumes. Compared to the 21.2 × 50 mm format, this configuration:
Provides increased resolving power and peak capacity
Improves separation of closely eluting compounds
Preserves chromatographic behavior during scale‑up
Balances throughput and resolution for routine preparative purification
The 100 mm length is ideal when additional separation confidence is required without the longer run times associated with 150 mm or 250 mm preparative columns.
Application Focus: Silica‑C™ for Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are selected for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for purification of:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry provides faster equilibration and moisture‑tolerant performance compared to conventional silica, increasing efficiency during preparative runs.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Excellent recovery at elevated preparative loadings
For HILIC separations, minimal salt is typically sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (21.2 mm ID): ~10–30 mL/min
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable