Prep HPLC Column, Silica-C, 4um, 100A. 21.2mm ID x 150mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P21‑150 Silica‑C™ Preparative HPLC Column is engineered for high‑capacity preparative purification and compound isolation when increased resolving power is required beyond shorter preparative formats. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers enhanced separation, fast equilibration, and robust performance for preparative HILIC and normal‑phase workflows.
The silica hydride surface chemistry provides moisture‑tolerant operation and stable retention behavior, supporting consistent yields and reliable turnaround in demanding preparative applications.
Preparative Use Case: Why 21.2 mm ID × 150 mm
The 21.2 mm internal diameter places this column in the full preparative class, supporting high sample loading and large fraction collection. Compared to 21.2 × 50 mm and 21.2 × 100 mm formats, this configuration:
Provides increased resolving power and peak capacity
Improves separation of closely eluting or structurally related compounds
Preserves chromatographic behavior during analytical‑to‑prep scale‑up
Balances throughput and resolution for routine preparative purification
The 150 mm column length is selected when additional separation confidence is required without the extended run times of 250 mm preparative columns.
Application Focus: Silica‑C™ for Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for preparative purification of:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry enables faster equilibration and moisture‑tolerant performance compared to conventional silica, improving productivity in preparative workflows.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Excellent recovery at elevated preparative loadings
For HILIC separations, minimal salt is often sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (21.2 mm ID): ~10–30 mL/min
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable