Prep HPLC Column, Silica-C, 4um, 100A. 21.2mm ID x 250mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P21‑250 Silica‑C™ Preparative HPLC Column is engineered for maximum‑resolution preparative purification and compound isolation when the highest possible separation of closely related analytes is required at scale. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers excellent recovery, fast equilibration, and robust performance for preparative HILIC and normal‑phase workflows.
The silica hydride surface chemistry provides moisture‑tolerant operation and stable retention behavior, supporting consistent yields and repeatable performance in demanding, large‑scale purification applications.
Preparative Use Case: Why 21.2 mm ID × 250 mm
The 21.2 mm internal diameter classifies this column as full preparative, supporting very high sample loading and large fraction collection volumes. Compared to 21.2 × 50 mm, 100 mm, and 150 mm formats, this configuration:
Provides maximum resolving power and peak capacity
Enables superior separation of closely related or structurally similar compounds
Delivers extended retention for challenging preparative separations
Preserves chromatographic behavior during analytical‑to‑preparative scale‑up
The 250 mm column length is selected when separation confidence and purity are critical, and longer run times are acceptable in exchange for superior resolution.
Application Focus: Silica‑C™ for Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for preparative purification of:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry enables faster equilibration and moisture‑tolerant performance compared to conventional silica, improving productivity and robustness in long preparative runs.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
High recovery at elevated preparative loadings
For HILIC separations, minimal salt is often sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (21.2 mm ID): ~10–30 mL/min
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable