CUSTOM ITEM: Prep HPLC Column, Silica-C, 5um, 300A. 21.2mm ID x 250mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P21‑25‑3M Silica‑C™ 300 Preparative HPLC Column is engineered for maximum‑resolution preparative purification of large molecules when both high loading capacity and extended pore accessibility are required. Manufactured with 5 µm, 300 Å Cogent TYPE‑C™ silica hydride, this column delivers high recovery, fast equilibration, and moisture‑tolerant performance for preparative HILIC and normal‑phase separations of macromolecules.
The silica hydride surface chemistry enables stable retention without moisture sensitivity, supporting consistent yields and reliable performance during long, demanding preparative runs.
Preparative Use Case: Why 21.2 mm ID × 250 mm, 300 Å
The 21.2 mm internal diameter classifies this column as full preparative, enabling very high sample loading and large fraction collection volumes. Combined with a 250 mm column length and 300 Å pore size, this format:
Provides maximum resolving power for macromolecules
Enables extended retention and peak capacity for closely related large compounds
Supports high‑purity isolation at preparative scale
Preserves chromatographic behavior during analytical‑to‑prep scale‑up
This configuration is selected when separation confidence and macromolecule accessibility are critical, and longer run times are acceptable to achieve maximum purity.
Application Focus: Silica‑C™ 300 for Preparative HILIC & Normal Phase
Silica‑C™ 300 preparative columns are chosen for large compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for preparative purification of:
Proteins
Large peptides
Oligonucleotides
Other macromolecules requiring wide pore access and non‑polar solvation
The TYPE‑C™ silica hydride chemistry provides moisture‑tolerant performance and reproducible retention, outperforming conventional silica in large‑molecule preparative workflows.
Key Advantages of Silica‑C™ 300 TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ 300 offers:
Faster equilibration between preparative runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under aggressive solvent conditions
Improved accessibility for large analytes due to wide pore structure
For HILIC separations, minimal salt is often sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (21.2 mm ID): ~10–30 mL/min
Requires a dedicated preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable