Prep HPLC Column, Silica-C, 4um, 100A. 21.2mm ID x 50mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P21‑50 Silica‑C™ Preparative HPLC Column is designed for high‑throughput preparative separations and compound purification when semi‑preparative formats no longer provide sufficient loading capacity or collection volume. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient recovery, fast equilibration, and robust performance for preparative HILIC and normal‑phase workflows.
The silica hydride surface provides moisture‑tolerant operation and stable retention behavior, supporting rapid turnaround and reliable yields during preparative purification.
Preparative Use Case: Why 21.2 mm ID × 50 mm
The 21.2 mm internal diameter classifies this column as full preparative, supporting substantially higher sample loading and larger fraction collection than 10 mm semi‑prep formats. Compared to shorter analytical and semi‑prep columns, this format:
Enables high‑load purification and large fraction volumes
Preserves chromatographic behavior during scale‑up
Transitions methods from development into routine preparative purification
The 50 mm column length emphasizes throughput and speed, making it ideal when rapid purification and turnaround are prioritized over maximum resolving power.
Application Focus: Silica‑C™ for Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for purifying:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry provides faster equilibration and moisture‑tolerant performance compared to conventional silica, improving productivity in preparative workflows.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Efficient recovery at higher preparative loadings
For HILIC separations, minimal salt is typically sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (21.2 mm ID): ~10–30 mL/min
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable