Prep HPLC Column, Silica-C, 4um, 100A. 30mm ID x 100mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P30‑100 Silica‑C™ Preparative HPLC Column is engineered for high‑capacity preparative purification when increased resolving power is required beyond short, throughput‑oriented preparative formats. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers robust recovery, fast equilibration, and moisture‑tolerant performance for large‑scale HILIC and normal‑phase separations.
The silica hydride surface chemistry provides stable retention without moisture sensitivity, supporting consistent yields and reliable throughput on dedicated large‑scale preparative HPLC systems.
Preparative Use Case: Why 30 mm ID × 100 mm
The 30 mm internal diameter places this column in the large‑scale preparative class, supporting very high sample loading and large fraction collection volumes. Compared to the 30 × 50 mm format, this configuration:
Provides increased resolving power and peak capacity
Improves separation of closely eluting compounds
Supports larger batch purification with maintained resolution
Balances throughput and separation confidence
The 100 mm column length is selected when additional resolution is required without the longer run times of 150 mm or 250 mm preparative columns.
Application Focus: Silica‑C™ for Large‑Scale Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for large‑scale purification of:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry enables faster equilibration and moisture‑tolerant performance compared to conventional silica, improving productivity in high‑capacity preparative workflows.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between preparative runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under aggressive solvent conditions
High recovery at elevated preparative loadings
For HILIC separations, minimal salt is often sufficient to elute analytes, simplifying downstream processing.
System Requirements
Typical operating flow rates (30 mm ID): ~20–60 mL/min
Requires a large‑scale preparative HPLC system capable of:
Sustained high‑flow delivery
Very large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable