Catalog Number - 40000-P30-150

The 40000‑P30‑150 Silica‑C™ Preparative HPLC Column is designed for high‑capacity, higher‑resolution preparative purification when additional resolving power is required beyond shorter 30 mm formats. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers excellent selectivity, faster equilibration, and robust column lifetime for preparative HILIC and normal‑phase separations.

The silica hydride surface chemistry provides moisture‑tolerant operation and stable retention behavior, enabling consistent recovery and reliable performance in demanding large‑scale purification workflows.

Preparative Use Case: Why 30 mm ID × 150 mm

The 30 mm internal diameter places this column in the large‑scale preparative class, supporting very high sample loading and large fraction collection volumes. Compared to 30 × 50 mm and 30 × 100 mm formats, this configuration:

• Provides increased resolving power and peak capacity

• Improves separation of closely eluting or structurally related analytes

• Supports larger batch purification with improved separation confidence

• Preserves chromatographic behavior during analytical‑to‑prep scale‑up

The 150 mm column length is selected when resolution and purity are prioritized, while maintaining manageable run times compared to 250 mm preparative columns.

Application Focus: Silica‑C™ for Large‑Scale Preparative HILIC & Normal Phase

Silica‑C™ preparative columns are chosen for purification (“prep”) of peptides, metabolites, polar compounds, and analytes with amine groups, particularly when large sample volumes must be isolated. This column is well suited for preparative purification of:

• Peptides

• Metabolites

• Polar and basic compounds

• Other analytes requiring non‑polar or minimally aqueous mobile phases

The TYPE‑C™ silica hydride chemistry provides faster equilibration and moisture‑tolerant performance compared to conventional silica, improving efficiency in extended preparative runs.

Key Advantages of Silica‑C™ TYPE‑C™ Technology

Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:

• Faster equilibration between preparative runs

• No need to control moisture in mobile‑phase solvents

• Robust column lifetime under aggressive solvent conditions

• High recovery at elevated preparative loadings

These attributes make Silica‑C™ a dependable solution for high‑confidence, large‑scale purification.

System Requirements

• Typical operating flow rates (30 mm ID): ~20–60 mL/min

• Requires a large‑scale preparative HPLC system capable of:

  • Sustained high‑flow delivery

  • Large injection volumes

  • Appropriate detectors and fraction‑collection hardware

Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.

Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.