Prep HPLC Column, Silica-C, 4um, 100A. 30mm ID x 250mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P30‑250 Silica‑C™ Preparative HPLC Column is engineered for maximum‑resolution, large‑scale preparative purification when the highest separation confidence is required at elevated loading levels. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers robust recovery, fast equilibration, and moisture‑tolerant performance for preparative HILIC and normal‑phase workflows on large‑bore systems.
The silica hydride surface chemistry provides stable retention without moisture sensitivity, supporting consistent yields and reproducible performance during long preparative runs.
Preparative Use Case: Why 30 mm ID × 250 mm
The 30 mm internal diameter places this column in the large‑scale preparative class, supporting very high sample loading and large fraction collection volumes. Compared to shorter 30 mm formats, this configuration:
Provides maximum resolving power and peak capacity within the 30 mm family
Enables extended retention for closely related or structurally similar compounds
Supports high‑purity isolation at preparative scale
Preserves chromatographic behavior during analytical‑to‑prep scale‑up
The 250 mm column length is selected when resolution and purity take priority and longer run times are acceptable in exchange for superior separation.
Application Focus: Silica‑C™ for Large‑Scale Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for large‑scale purification of:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry enables faster equilibration and moisture‑tolerant operation compared to conventional silica, improving productivity and reliability in extended preparative workflows.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between preparative runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under aggressive solvent conditions
High recovery at elevated preparative loadings
For HILIC separations, minimal salt is often sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (30 mm ID): ~20–60 mL/min
Requires a large‑scale preparative HPLC system capable of:
Sustained high‑flow delivery
Very large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable