Prep HPLC Column, Silica-C, 4um, 100A. 30mm ID x 50mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P30‑50 Silica‑C™ Preparative HPLC Column is engineered for large‑bore, high‑throughput preparative purification when maximum loading capacity and rapid turnaround are required. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers efficient recovery, fast equilibration, and robust performance for preparative HILIC and normal‑phase workflows at elevated flow rates.
The silica hydride surface chemistry provides moisture‑tolerant operation and stable retention behavior, supporting consistent yields in demanding, high‑capacity preparative applications.
Preparative Use Case: Why 30 mm ID × 50 mm
The 30 mm internal diameter places this column in the large‑scale preparative class, supporting very high sample loading and large fraction collection volumes beyond 21.2 mm formats. Compared to smaller‑ID preparative columns, this configuration:
Enables maximum throughput and loading capacity
Supports large injection volumes for rapid compound isolation
Preserves chromatographic behavior during scale‑up
Transitions methods into production‑oriented purification workflows
The 50 mm column length emphasizes speed and throughput, making this format ideal when rapid purification and turnaround are prioritized over maximum resolving power.
Application Focus: Silica‑C™ for Large‑Scale Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for large‑scale purification of:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry provides faster equilibration and moisture‑tolerant performance compared to conventional silica, increasing productivity in high‑throughput preparative runs.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under aggressive solvent systems
High recovery at elevated preparative loadings
For HILIC separations, minimal salt is often sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (30 mm ID): ~20–60 mL/min
Requires a large‑scale preparative HPLC system capable of:
Sustained high‑flow delivery
Very large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable