Prep HPLC Column, Silica-C, 10um, 100A. 50mm ID x 50mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑P50‑50 Silica‑C™ Preparative HPLC Column is engineered for very high‑capacity, large‑scale preparative purification where maximum loading and rapid turnaround are critical. Manufactured with 10 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers efficient recovery, fast equilibration, and moisture‑tolerant performance for preparative HILIC and normal‑phase workflows on large‑bore systems.
The silica hydride surface chemistry provides stable retention without moisture sensitivity, supporting consistent yields and reproducible performance during high‑throughput, production‑oriented purification.
Preparative Use Case: Why 50 mm ID × 50 mm
The 50 mm internal diameter places this column in the large‑scale / production preparative class, supporting extremely high sample loading and very large fraction collection volumes beyond 21.2 mm and 30 mm formats. Compared to smaller‑bore preparative columns, this configuration:
Enables maximum loading capacity and throughput
Supports very large injection volumes for rapid isolation
Preserves chromatographic behavior during scale‑up
Transitions methods into production‑oriented purification workflows
The 50 mm column length emphasizes speed and throughput, making this format ideal when rapid purification and turnaround are prioritized over maximum resolving power.
Application Focus: Silica‑C™ for Large‑Scale Preparative HILIC & Normal Phase
Silica‑C™ preparative columns are selected for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for large‑scale purification of:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride chemistry enables faster equilibration and moisture‑tolerant operation compared to conventional silica, increasing productivity in high‑throughput preparative runs.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica preparative columns, Silica‑C™ offers:
Faster equilibration between preparative runs
No need to control moisture in mobile‑phase solvents
Robust column lifetime under aggressive solvent conditions
High recovery at very high preparative loadings
For HILIC separations, minimal salt is often sufficient to elute analytes, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow ranges (50 mm ID): ~60–150 mL/min (system‑dependent)
Requires a large‑scale/production preparative HPLC system capable of:
Sustained very high‑flow delivery
Very large injection volumes
Appropriate detectors and fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 50 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable