Semi Prep HPLC Column, Silica-C, 4um, 100A. 10mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑SP100 Silica‑C™ Semi‑Preparative HPLC Column is designed for method scale‑up and compound isolation when increased sample loading and recovery are required beyond analytical formats. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers efficient recovery, fast equilibration, and robust performance for semi‑preparative HILIC and normal‑phase separations.
The silica hydride surface enables moisture‑tolerant operation and rapid re‑equilibration, supporting higher throughput and consistent yields in semi‑prep workflows.
Semi‑Preparative Use Case: Why 10 mm ID × 100 mm
The 10 mm internal diameter classifies this column as semi‑preparative, providing a practical bridge between analytical HPLC and full preparative purification. Compared to 4.6 mm analytical columns, this format:
Supports significantly higher sample loading
Enables collection of larger fractions
Preserves chromatographic behavior during scale‑up
Bridges analytical method development and preparative workflows
The 100 mm column length provides additional resolving power compared to 50 mm semi‑prep formats, improving separation of closely eluting compounds while maintaining efficient run times.
Application Focus: Silica‑C™ for Semi‑Preparative HILIC & Normal Phase
Silica‑C™ semi‑preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other compounds requiring non‑polar solvents or HILIC conditions
The TYPE‑C™ silica hydride surface provides faster equilibration and stable retention, increasing productivity during semi‑prep isolations.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to conventional bonded‑silica semi‑prep columns, Silica‑C™ offers:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Improved recovery and reproducibility at higher loadings
For HILIC separations, minimal salt is typically sufficient to elute analytes, simplifying downstream processing.
System Requirements
Typical operating flow rates (10 mm ID): ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Higher flow delivery
Increased injection volumes
Appropriate detection and fraction‑collection support
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable