Semi Prep HPLC Column, Silica-C, 4um, 100A. 10mm ID x 150mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑SP150 Silica‑C™ Semi‑Preparative HPLC Column is designed for higher‑resolution method scale‑up and compound isolation when additional resolving power is required beyond shorter semi‑prep formats. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers efficient recovery, fast equilibration, and robust performance for semi‑preparative HILIC and normal‑phase separations.
The silica hydride surface provides moisture‑tolerant operation and stable retention behavior, supporting consistent yields and reliable turnaround times in semi‑preparative workflows.
Semi‑Preparative Use Case: Why 10 mm ID × 150 mm
The 10 mm internal diameter classifies this column as semi‑preparative, making it ideal for isolations requiring higher loading than analytical columns while avoiding full preparative scale. Compared to 10 × 50 mm and 10 × 100 mm formats, this configuration:
Provides increased resolving power and peak capacity
Supports higher sample loading for complex mixtures
Preserves chromatographic behavior during scale‑up
Bridges analytical development and preparative purification
The 150 mm column length is selected when additional separation confidence is required for closely eluting analytes, while maintaining reasonable run times and throughput.
Application Focus: Silica‑C™ for Semi‑Preparative HILIC & Normal Phase
Silica‑C™ semi‑preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other compounds requiring non‑polar solvents or HILIC conditions
The TYPE‑C™ silica hydride surface enables faster equilibration and stable retention, improving productivity during higher‑resolution semi‑prep isolations.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to conventional bonded‑silica semi‑prep columns, Silica‑C™ offers:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Improved recovery and reproducibility at higher loadings
For HILIC separations, using minimal salt is typically sufficient to elute analytes, simplifying downstream purification and solvent removal.
System Requirements
Typical operating flow rates (10 mm ID): ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Higher flow delivery
Increased injection volumes
Appropriate detection and fraction‑collection support
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable