Semi Prep HPLC Column, Silica-C, 4um, 100A. 10mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑SP250 Silica‑C™ Semi‑Preparative HPLC Column is designed for maximum‑resolution semi‑preparative separations and compound isolation when closely related analytes require extended retention and higher peak capacity. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers robust recovery, moisture‑tolerant operation, and fast equilibration for demanding semi‑prep HILIC and normal‑phase workflows.
The silica hydride surface chemistry enables consistent retention behavior without moisture sensitivity, making this column ideal for high‑confidence isolations where separation quality is prioritized over total run time.
Semi‑Preparative Use Case: Why 10 mm ID × 250 mm
The 10 mm internal diameter places this column firmly in the semi‑preparative class, supporting significantly higher sample loading than analytical columns while remaining compatible with semi‑prep HPLC systems. Compared to shorter 10 mm formats, the 250 mm length provides:
Maximum resolving power and peak capacity within the semi‑prep family
Extended retention for closely related or structurally similar compounds
Higher confidence fraction purity
Preserved chromatographic behavior during scale‑up
This format is selected when resolution and isolation confidence are critical, even at the cost of longer run times.
Application Focus: Silica‑C™ for Semi‑Preparative HILIC & Normal Phase
Silica‑C™ semi‑preparative columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for isolating:
Lipids
Peptides and proteins
Oligonucleotides
Other non‑polar or weakly water‑soluble compounds
The TYPE‑C™ silica hydride surface provides stable retention and faster equilibration compared to conventional bonded silica, increasing productivity even in long‑column semi‑prep runs.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica semi‑preparative columns, Silica‑C™ offers:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under aggressive solvent conditions
Improved recovery and reproducibility at elevated loadings
In HILIC workflows, minimal salt is typically sufficient for elution, simplifying downstream processing and solvent removal.
System Requirements
Typical operating flow rates (10 mm ID): ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Sustained higher‑flow delivery
Larger injection volumes
Appropriate detection and fraction‑collection hardware
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Final flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and isolation objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable