Semi Prep HPLC Column, Silica-C, 4um, 100A. 10mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑SP50 Silica‑C™ Semi‑Preparative HPLC Column is designed for method scale‑up and compound isolation when analytical‑scale columns no longer provide sufficient sample loading or recovery. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column delivers efficient recovery, fast equilibration, and robust performance for semi‑preparative HILIC and normal‑phase separations.
The silica hydride surface enables stable retention and rapid equilibration, making this column well suited for high‑throughput semi‑prep workflows where turnaround time and yield are prioritized.
Semi‑Preparative Use Case: Why 10 mm ID × 50 mm
The 10 mm internal diameter classifies this column as semi‑preparative, providing a practical bridge between analytical HPLC and full preparative purification. Compared to 4.6 mm analytical columns, this format:
Supports significantly higher sample loading
Enables collection of larger fractions
Preserves chromatographic behavior during scale‑up
Bridges analytical method development and preparative purification
The 50 mm column length emphasizes speed and throughput, allowing rapid separations while maintaining sufficient resolution for targeted isolation of non‑polar and weakly water‑soluble compounds.
Application Focus: Silica‑C™ for Semi‑Preparative HILIC & Normal Phase
Silica‑C™ semi‑prep columns are chosen for compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. This column is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other compounds requiring non‑polar solvents or HILIC conditions
The TYPE‑C™ silica hydride surface provides faster equilibration and moisture‑tolerant performance compared to conventional silica, improving productivity during semi‑prep runs.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica semi‑prep columns, Silica‑C™ offers:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Improved recovery and reproducibility at higher loadings
For HILIC separations, use of minimal salt is often sufficient to elute analytes, simplifying downstream processing.
System Requirements
Typical operating flow rates (10 mm ID): ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Higher flow delivery
Increased injection volumes
Appropriate detection and fraction‑collection support
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable