HPLC Column, Bidentate C18, 4um, 4.6mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑10P Cogent Bidentate C18™ HPLC Column is an analytical‑scale reversed‑phase column designed for separations that require greater resolving power than short (50 mm) columns, but without the long run times associated with very long column formats. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers unique selectivity, fast equilibration, and exceptional chemical robustness.
The stationary phase uses an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry creates a highly stable, hydrophobic phase that behaves differently from conventional silica‑based C18 columns—retaining compounds that often show poor or inconsistent retention on standard bonded phases.
Unlike traditional C18 columns, the Bidentate C18™ can be operated with 100 % aqueous mobile phases without phase collapse and can tolerate very low pH conditions (below pH 2) with no damage to the column. These properties make it suitable for methods that challenge conventional reversed‑phase technologies.
The 100 mm column length provides increased plate count and separation efficiency compared to 50 mm formats, making this column a preferred choice when additional resolution is required but overall analysis time must remain practical.
Why Choose the 100 mm Bidentate C18™ Format
The 4.6 × 100 mm configuration is commonly selected when:
50 mm columns do not provide sufficient resolution
Closely eluting compounds require additional separation space
Method development calls for increased selectivity control
Run time must remain manageable on standard analytical systems
When combined with TYPE‑C™ silica hydride chemistry, the 100 mm length maintains fast equilibration and long service life even under aggressive method conditions.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional silica‑based C18 columns
Extremely robust column lifetime
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Unique selectivity not achievable with traditional C18 phases
Retains hydrophobic compounds and select polar analytes
Exceptional Mobile‑Phase Flexibility
Compatible with 100 % aqueous mobile phases
Stable at very low pH (below 2)
Suitable for unconventional or aggressive reversed‑phase methods
Analytical‑Scale Performance
4.6 mm ID × 100 mm length
Increased resolving power vs. 50 mm columns
Compatible with standard analytical HPLC instrumentation
Typical Applications
Analytical HPLC requiring enhanced resolution
Compounds poorly retained on conventional C18 columns
Low‑pH reversed‑phase separations
Aqueous‑dominant mobile‑phase methods
Method development and optimization