HPLC Column, Bidentate C18, 4um, 2.1mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑10P‑2 Cogent Bidentate C18™ HPLC Column is a high‑resolution, narrow‑bore reversed‑phase column designed for LC‑MS applications where additional resolving power is required beyond short (50 mm) formats. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers unique selectivity, rapid equilibration, and exceptional chemical robustness compared to conventional silica‑based C18 columns.
The stationary phase features an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is highly hydrophobic yet versatile, capable of retaining compounds that often show poor retention on traditional C18 columns. Unlike standard C18 phases, this column can be operated with 100 % aqueous mobile phases without phase collapse and can tolerate very low pH conditions (below pH 2) with no damage.
The 2.1 mm internal diameter enhances MS sensitivity while lowering solvent consumption, and the 100 mm length increases plate count and separation efficiency. This combination makes the column a popular choice for LC‑MS workflows when increased resolution is needed for complex or closely eluting analytes.
Why Choose the 2.1 × 100 mm Bidentate C18™ Format
This column size is commonly selected when:
50 mm LC‑MS columns do not provide sufficient resolution
Additional separation space is required for complex mixtures
Improved sensitivity and reduced solvent use are desired
Methods operate at low pH or with aqueous‑dominant mobile phases
When paired with TYPE‑C™ silica hydride chemistry, the longer length delivers higher resolution without sacrificing equilibration speed or column lifetime.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional C18 columns
Extremely robust column lifetime
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Unique selectivity not achievable with standard C18 phases
Retains hydrophobic compounds and select polar analytes
LC‑MS Optimized Geometry
2.1 mm ID enhances MS sensitivity and reduces solvent use
100 mm length increases resolving power vs. 50 mm formats
Well suited for ESI‑MS and LC‑MS/MS workflows
Broad Mobile‑Phase Compatibility
Stable with 100 % aqueous mobile phases
Operable at very low pH (below 2)
Suitable for aggressive reversed‑phase methods
System Requirements
Due to the narrow‑bore format, optimal performance requires:
A pump capable of precise, reproducible low‑flow delivery
A detector flow cell designed for reduced internal volume
Typical Applications
LC‑MS and LC‑MS/MS
High‑resolution reversed‑phase separations
Hydrophobic compounds poorly retained on conventional C18 columns
Low‑pH analytical methods
Method development and optimization