HPLC Column, Bidentate C18, 4um, 2.1mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑15P‑2 Cogent Bidentate C18™ HPLC Column is a long‑format, narrow‑bore analytical column designed for LC‑MS workflows requiring maximum resolving power. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers unique selectivity, rapid equilibration, and exceptional chemical robustness compared with conventional silica‑based C18 columns.
The stationary phase employs an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry yields a phase that is highly hydrophobic yet versatile, retaining compounds that often show weak or inconsistent retention on standard C18 phases. The column can operate with 100 % aqueous mobile phases without phase collapse and tolerates very low pH conditions (below pH 2) without degradation.
The 2.1 mm internal diameter enhances MS sensitivity while lowering solvent consumption, and the 150 mm length provides maximum plate count for separating closely eluting or complex mixtures. This configuration is frequently chosen when increased resolution is required beyond 100 mm LC‑MS columns.
Why Choose the 2.1 × 150 mm Bidentate C18™ Format
This format is ideal when:
LC‑MS methods demand highest resolution
Complex samples require additional separation space
Sensitivity and solvent efficiency are priorities
Methods utilize aqueous‑dominant mobile phases or low pH
Combined with TYPE‑C™ silica hydride chemistry, the longer length maintains fast equilibration and long column life—even under aggressive conditions.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional C18 columns
Extremely robust column lifetime
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Unique selectivity not achievable with traditional C18 phases
Retains hydrophobic compounds and select polar analytes
LC‑MS Optimized Geometry
2.1 mm ID increases MS sensitivity and reduces solvent use
150 mm length maximizes resolving power
Suitable for ESI‑MS and LC‑MS/MS workflows
Broad Mobile‑Phase Compatibility
Stable with 100 % aqueous mobile phases
Operable at very low pH (below 2)
Supports aggressive reversed‑phase methods
System Requirements
For optimal performance with this narrow‑bore, long‑format column:
Use a pump capable of precise, stable low‑flow delivery
Employ reduced‑volume detector flow cells appropriate for 2.1 mm ID columns
Typical Applications
LC‑MS and LC‑MS/MS requiring high resolution
Complex or closely eluting analyte separations
Hydrophobic compounds poorly retained on conventional C18
Low‑pH reversed‑phase methods
Method development and optimization