Prep HPLC Column, Bidentate C18, 4um, 100A. 21.2mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑P21‑100 Cogent Bidentate C18™ Preparative HPLC Column is a longer preparative‑scale column designed for applications that require greater separation efficiency and higher purity fractions than short‑length prep formats. Manufactured using Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetime under demanding preparative conditions.
The stationary phase employs an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is highly hydrophobic yet versatile, providing strong, stable retention of hydrophobic molecules, metabolites, and amine‑containing analytes. Unlike conventional silica‑based prep C18 columns, the TYPE‑C™ hydride surface remains stable in aqueous‑dominant mobile phases and tolerates low‑pH conditions, expanding method flexibility for purification workflows.
With a 21.2 mm internal diameter and an extended 100 mm length, this column offers increased plate count and improved resolution compared with 21.2 × 50 mm formats, making it well suited when closely related components must be better separated prior to fraction collection.
System Note: A specialized preparative HPLC system (appropriate high‑flow pump capacity, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size.
Why Choose the 21.2 × 100 mm Prep Bidentate C18™ Format
This configuration is commonly selected when:
Higher purity fractions are required than short prep columns can deliver
Sample loads are large and contain closely eluting impurities
Hydrophobic or basic analytes challenge traditional prep C18 materials
Fast equilibration and robustness are essential in repeated prep runs
The longer length increases interaction time and separation efficiency while maintaining the durability and flexibility of TYPE‑C™ silica hydride chemistry.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional prep C18 columns
Very robust lifetimes for repeated purification cycles
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Strong hydrophobic retention with versatile selectivity
Suitable for hydrophobic and amine‑containing analytes
Preparative Geometry with Enhanced Resolution
21.2 mm ID × 100 mm length
Higher plate count vs. 21.2 × 50 mm columns
Supports improved purification and tighter fraction cuts
Broad Operating Window
Compatible with aqueous‑dominant mobile phases
Stable under low‑pH conditions
Tolerates aggressive purification methods
Typical Applications
Preparative purification and fraction collection
Hydrophobic molecule and metabolite isolation
Amine‑containing analytes
Scale‑up from analytical or shorter preparative methods
Workflows requiring higher purity or improved separation
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable