Prep HPLC Column, Bidentate C18, 4um, 100A. 21.2mm ID x 150mm Length. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 40018‑P21‑150 Cogent Bidentate C18™ Preparative HPLC Column is a long‑format preparative column designed for workflows that require higher resolving power and improved fraction purity compared with shorter preparative lengths. Built on Cogent TYPE‑C™ silica hydride technology, this column provides excellent selectivity, fast equilibration, and very robust column lifetimes under demanding purification conditions.

The stationary phase utilizes an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This non‑traditional reversed‑phase chemistry creates a phase that is highly hydrophobic yet versatile, enabling strong and stable retention of hydrophobic molecules, metabolites, and amine‑containing analytes. In contrast to conventional silica‑based prep C18 columns, the TYPE‑C™ hydride surface remains stable in aqueous‑dominant mobile phases and tolerates low‑pH conditions, expanding method flexibility for preparative workflows.

With a 21.2 mm internal diameter and an extended 150 mm length, this column delivers increased plate count and enhanced separation efficiency, making it especially useful when closely eluting impurities must be fully resolved prior to fraction collection.

System Note: A specialized preparative HPLC system (appropriate high‑flow pump capacity, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size and length.

Why Choose the 21.2 × 150 mm Prep Bidentate C18™ Format

This configuration is commonly selected when:

  • Higher purification resolution is required than 50 mm or 100 mm prep columns can provide

  • Sample loads are large and contain closely related components

  • Hydrophobic or basic analytes challenge traditional prep C18 materials

  • Method robustness and reproducibility are critical across repeated prep cycles

The longer length increases interaction time and selectivity while preserving the durability and rapid equilibration inherent to TYPE‑C™ silica hydride chemistry.

Key Features

Cogent TYPE‑C™ Silica Hydride Technology

  • Reduced silanol activity and secondary interactions

  • Faster equilibration than conventional prep C18 columns

  • Very robust lifetimes for repeated purification cycles

Bidentate C18™ Bonding

  • Two‑point attachment via direct Si–C bonds

  • Strong hydrophobic retention with versatile selectivity

  • Suitable for hydrophobic and amine‑containing analytes

Preparative Geometry with Enhanced Resolution

  • 21.2 mm ID × 150 mm length

  • Higher plate count vs. shorter prep formats

  • Supports tighter fraction cuts and higher purity outcomes

Broad Operating Window

  • Compatible with aqueous‑dominant mobile phases

  • Stable under low‑pH conditions

  • Tolerates aggressive purification methods

Typical Applications

  • Preparative purification and fraction collection

  • Hydrophobic molecule and metabolite isolation

  • Amine‑containing analytes

  • Scale‑up from analytical or shorter preparative methods

  • Workflows requiring higher purity separations


Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

  • Orders cannot be canceled once placed

  • Custom items are non‑returnable

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