Prep HPLC Column, Bidentate C18, 4um, 100A. 21.2mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 40018‑P21‑50 Cogent Bidentate C18™ Preparative HPLC Column is a short‑length, large‑diameter prep column designed for high‑throughput purification and efficient fraction collection. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetime under preparative operating conditions.

The stationary phase employs an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This non‑traditional chemistry produces a phase that is highly hydrophobic yet versatile, enabling strong and stable retention of hydrophobic molecules, metabolites, and amine‑containing analytes—including in aqueous‑dominant or low‑pH mobile phases that can challenge conventional silica‑based prep C18 materials.

With a 21.2 mm internal diameter and 50 mm length, this format is optimized for large injection volumes and high mass loads, making it ideal when rapid purification cycles and collection efficiency are prioritized over extended resolving power.

System Note: A specialized preparative HPLC system (appropriate high‑flow pump capacity, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size.

Why Choose the 21.2 × 50 mm Prep Bidentate C18™ Format

This configuration is commonly selected when:

  • Large sample loads must be purified quickly

  • Hydrophobic or basic (amine‑containing) analytes require strong, stable retention

  • Fast equilibration and throughput are critical

  • Fractions are collected at preparative scale with minimal cycle time

The short length supports rapid runs, while TYPE‑C™ silica hydride chemistry maintains stability and reproducibility across aggressive prep methods.

Key Features

Cogent TYPE‑C™ Silica Hydride Technology

  • Reduced silanol activity and secondary interactions

  • Faster equilibration than conventional prep C18 columns

  • Very robust lifetimes for repeated purification cycles

Bidentate C18™ Bonding

  • Two‑point attachment via direct Si–C bonds

  • Strong hydrophobic retention with versatile selectivity

  • Suitable for hydrophobic molecules and amine‑containing analytes

Preparative Geometry for Throughput

  • 21.2 mm ID × 50 mm length

  • High mass loading and efficient fraction collection

  • Short cycle times for rapid purification

Broad Operating Window

  • Compatible with aqueous‑dominant mobile phases

  • Stable under low‑pH conditions

  • Tolerates aggressive purification methods

Typical Applications

  • Preparative purification and fraction collection

  • Hydrophobic molecule isolation

  • Metabolite preparation

  • Amine‑containing analytes

  • Scale‑up from analytical or semi‑prep methods

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

  • Orders cannot be canceled once placed

  • Custom items are non‑returnable

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