Semi Prep HPLC Column, Bidentate C18, 4um, 100A. 10mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑SP100 Cogent Bidentate C18™ Semi‑Preparative HPLC Column is a longer, high‑capacity purification column designed for applications that require greater resolving power than 50 mm semi‑prep formats while maintaining excellent throughput and robustness. Built on Cogent TYPE‑C™ silica hydride technology, this column offers excellent selectivity, fast equilibration, and very long column lifetime under demanding purification conditions.
The stationary phase employs an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry creates a phase that is highly hydrophobic yet versatile, providing strong retention of hydrophobic molecules, metabolites, and amine‑containing analytes—even under aqueous‑dominant or low‑pH mobile‑phase conditions where conventional silica‑based prep C18 columns can struggle.
With a 10 mm internal diameter and an extended 100 mm length, this column delivers increased plate count and improved separation during semi‑preparative runs, making it ideal when higher purity or better resolution between closely eluting components is required prior to fraction collection.
System Note: A specialized semi‑preparative HPLC system (appropriate pump flow capacity, tubing, injector, and fraction‑collection hardware) is required for columns of this size.
Why Choose the 10 × 100 mm Semi‑Prep Bidentate C18™ Format
This configuration is typically selected when:
Greater resolution is needed than 50 mm semi‑prep columns can provide
Larger sample volumes must be purified with improved selectivity
Hydrophobic or basic analytes challenge traditional prep C18 materials
Fast equilibration and method robustness are critical
The longer length increases interaction time and separation efficiency while preserving the inherent durability and flexibility of TYPE‑C™ silica hydride chemistry.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Faster equilibration than conventional prep C18 columns
Very robust lifetimes for repeated purification cycles
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Strong, stable hydrophobic retention
Suitable for hydrophobic and amine‑containing analytes
Semi‑Preparative Geometry for Enhanced Resolution
10 mm ID × 100 mm length
Higher plate count vs. 50 mm semi‑prep columns
Supports efficient purification and fraction collection
Broad Operating Window
Compatible with aqueous‑dominant mobile phases
Stable at low pH
Tolerates aggressive purification methods
Typical Applications
Semi‑preparative purification
Fraction collection with higher purity requirements
Hydrophobic molecule isolation
Metabolite cleanup and enrichment
Preparative workflows following analytical method transfer
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable