Semi Prep HPLC Column, Bidentate C18, 4um, 100A. 10mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑SP250 Cogent Bidentate C18™ Semi‑Preparative HPLC Column is the longest and highest‑resolving semi‑prep format in the Bidentate C18™ lineup, designed for purification workflows that demand maximum separation, extended retention, and the highest achievable fraction purity. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetimes under repetitive purification conditions.
The stationary phase employs an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This unique chemistry creates a phase that is highly hydrophobic yet versatile, enabling strong and stable retention of hydrophobic molecules, metabolites, and amine‑containing analytes—even under aqueous‑dominant or low‑pH mobile‑phase conditions where conventional semi‑prep C18 columns often struggle.
With a 10 mm internal diameter and an extended 250 mm length, this column delivers the highest plate count available in a 10 mm semi‑preparative format, making it the preferred choice when closely eluting impurities must be fully resolved prior to fraction collection.
System Note: A specialized semi‑preparative HPLC system (high‑flow pump capacity, appropriate tubing/valving, injector, and fraction‑collection hardware) is required for columns of this size and length.
Why Choose the 10 × 250 mm Semi‑Prep Bidentate C18™ Format
This configuration is typically selected when:
Maximum purification resolution is required
50 mm, 100 mm, or 150 mm semi‑prep columns do not provide adequate separation
Large sample loads contain closely related impurities or isomers
Highest fraction purity is required before downstream processing
The extended column length dramatically increases interaction time and selectivity, while TYPE‑C™ silica hydride chemistry maintains fast equilibration and long service life despite aggressive prep conditions.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Faster equilibration than conventional prep C18 columns
Very robust lifetimes for repeated purification cycles
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Strong, stable hydrophobic retention
Particularly effective for hydrophobic and basic analytes
Semi‑Preparative Geometry for Maximum Resolution
10 mm ID × 250 mm length
Highest plate count among 10 mm semi‑prep options
Supports demanding purification and tight fraction cut windows
Broad Operating Window
Compatible with aqueous‑dominant mobile phases
Stable under low‑pH conditions
Tolerates aggressive purification methods
Typical Applications
Semi‑preparative purification with maximum resolution needs
Isolation of closely related or structurally similar compounds
Hydrophobic molecule and metabolite purification
Amine‑containing analytes
Scale‑up from analytical or shorter semi‑prep methods
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable