Semi Prep HPLC Column, Bidentate C18, 4um, 100A. 10mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑SP50 Cogent Bidentate C18™ Semi‑Preparative HPLC Column is a short‑length, large‑bore purification column engineered for high sample loads and efficient fraction collection. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetime under semi‑preparative conditions.
The stationary phase uses an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry creates a phase that is highly hydrophobic yet versatile, enabling strong retention of hydrophobic molecules while remaining tolerant of aggressive mobile phases. Unlike conventional silica‑based C18 materials, the TYPE‑C™ hydride surface supports aqueous‑dominant mobile phases and low‑pH operation with minimal risk of degradation.
With a 10 mm internal diameter and 50 mm length, this column is optimized for purification (“prep”) of hydrophobic molecules, metabolites, and amine‑containing analytes where larger injection volumes and high throughput are required.
System Note: A specialized semi‑preparative HPLC system (higher flow capability, appropriate tubing/valving, and fraction collection hardware) is required for columns of this size.
Why Choose a 10 mm Semi‑Prep Bidentate C18™ Column
This format is selected when:
Target compounds must be isolated and collected (not just analyzed)
High mass loading and recovery are required
Fast equilibration and method robustness are priorities
Hydrophobic or basic/amine‑containing analytes challenge conventional prep C18 phases
The short 50 mm length supports rapid cycles between injections, while the large bore enables efficient purification at preparative flow rates.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Faster equilibration than conventional C18 prep columns
Very robust lifetime for repeated prep runs
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Strong hydrophobic retention with versatile selectivity
Suitable for hydrophobic molecules and amines
Semi‑Preparative Geometry
10 mm ID × 50 mm length
Supports larger injection volumes and higher mass loads
Efficient fraction collection with short cycle times
Broad Method Compatibility
Compatible with aqueous‑dominant mobile phases
Stable under low‑pH conditions
Suitable for demanding purification workflows
Typical Applications
Semi‑preparative purification
Fraction collection of hydrophobic molecules
Metabolite isolation
Basic and amine‑containing analytes
Method scale‑up from analytical development
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable