Catalog Number - 40031-15P-2

40031-15P-2 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering a uniquely powerful mixed‑mode separation mechanism that cannot be replicated with traditional reversed phase or standalone ion exchange columns.

The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, which enables:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This multi‑mode behavior allows highly effective separation of basic, polar, and ionizable analytes, particularly in complex matrices where traditional methods fail to resolve co‑eluting compounds.

The TYPE‑C silica hydride base provides critical advantages:

• Direct silicon–carbon bonding for exceptional chemical stability

• Operation at very low pH (<2) without damage

• Faster equilibration times versus conventional silica phases

• Reduced sensitivity to mobile phase moisture in HILIC

150 mm Length – Maximum Resolution for Complex LC‑MS Workflows

The 150 mm length offers the highest resolving power in this format, ideal for challenging separations.

• Highest resolution compared to 50 mm and 100 mm formats

• Improved separation of structurally similar compounds

• Increased peak capacity for complex matrices

✅ Recommended for LC‑MS workflows requiring maximum resolution and analytical precision

Flow Rate & System Requirements (2.1 mm ID, 4 µm)

⚠️ Requires low‑flow capable HPLC or UHPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Moderate backpressure compared to sub‑2 µm columns

• Requires precise, stable low‑flow solvent delivery

• Optimized for low‑dispersion systems and small flow cells

✅ Important: Standard HPLC systems must be capable of accurate low‑flow operation

✅ System Fit: Ideal for LC‑MS platforms and modern analytical systems

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves co‑eluting and structurally similar compounds

• Effective for basic and positively charged analytes

pH‑Driven Selectivity Control

• WCX mechanism active at pH ≥ 6

• Retention tunable via pH and ionic strength

✅ Ideal when C18, phenyl, or standard ion exchange columns fail to provide adequate selectivity

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under harsh conditions including very low pH

• Resistant to hydrolysis

Faster Equilibration

• Reduces total method cycle time

No Strict Moisture Control (HILIC)

• Simplifies method development

Long Column Lifetime

• Durable for demanding analytical use

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in a single column

Balanced Performance (4 µm Particles)

• Lower pressure than UHPLC columns

• Broader system compatibility

LC‑MS Optimized

• Minimal salt requirements in HILIC mode

• Improved MS sensitivity and reduced contamination

Typical Applications

• LC‑MS method development

• Complex matrix separations

• Basic and positively charged analytes

• Polar and ionizable compounds

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires low‑flow capable HPLC or UHPLC system

• Suitable for both hydrophilic and hydrophobic analytes

• pH control is critical to modulate WCX selectivity