Catalog Number - 40031-15P-3

40031-15P-3 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering a uniquely powerful mixed‑mode separation mechanism that combines reversed phase, HILIC, and ion exchange selectivity.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling multiple retention mechanisms:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This multi‑mode behavior is especially effective for basic, polar, and ionizable compounds, making it ideal for complex matrices where conventional columns (e.g. C18) lack selectivity.

The TYPE‑C silica hydride base provides significant advantages:

• Direct silicon–carbon bonding for superior durability

• Stability at very low pH (<2) without degradation

• Faster equilibration vs traditional silica

• Reduced sensitivity to mobile phase moisture in HILIC

150 mm Length – High Resolution with Solvent Efficiency

The 150 mm length offers enhanced resolving power while maintaining reduced solvent usage due to the 3.0 mm ID format.

• Higher resolution vs 50 mm and 100 mm formats

• Improved separation of closely related compounds

• Increased peak capacity for complex matrices

✅ Recommended for methods requiring high resolution with lower solvent consumption

3.0 mm ID – Solvent Saver Format

This 3.0 mm ID column bridges the gap between standard 4.6 mm HPLC and 2.1 mm UHPLC formats.

• Similar run times as 4.6 mm ID columns

• Reduced solvent consumption and cost

• Lower flow rates while maintaining performance

✅ Ideal for labs seeking cost savings without requiring UHPLC systems

Flow Rate & System Requirements (3.0 mm ID, 4 µm)

✅ Compatible with most standard HPLC systems

• Typical flow rate range: ~0.4 – 0.8 mL/min

• Moderate backpressure

• No special UHPLC system required

• Compatible with UV and MS detectors

✅ Best Practice: Use appropriate filtration and guard columns to maximize column life

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves co‑eluting and structurally similar compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX functionality active at pH ≥ 6

• Tunable retention via pH and ionic strength

✅ Ideal when C18, phenyl, or traditional ion exchange columns are not sufficient

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under low pH conditions (<2)

• Resistant to hydrolysis

Faster Equilibration

• Improves throughput and reduces downtime

No Strict Moisture Control (HILIC)

• Easier method development

Extended Column Lifetimes

• Durable for routine and demanding applications

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Solvent Reduction (3.0 mm ID)

• Lower operating cost vs 4.6 mm columns

Balanced Performance (4 µm Particles)

• Compatible with standard HPLC systems

• Lower pressure vs UHPLC columns

Typical Applications

• Complex matrix separations

• LC‑MS method development

• Basic and positively charged compounds

• Polar and ionizable analytes

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Compatible with standard HPLC systems

• Designed for reduced solvent consumption workflows

• pH control is important for WCX selectivity

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.