Catalog Number - 40031-25P-3

40031-25P-3 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering powerful and highly differentiated selectivity through a true mixed‑mode mechanism.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid functional group, enabling:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This multi‑mode retention system makes the column exceptionally effective for polar, ionizable, and basic compounds, especially in complex matrices where traditional C18 or ion exchange columns fail to resolve co‑eluting peaks.

The TYPE‑C silica hydride base provides critical performance advantages:

• Direct silicon–carbon bonding for superior chemical stability

• Ability to operate at very low pH (<2) without damage

• Faster equilibration compared to conventional silica columns

• Reduced sensitivity to moisture in mobile phases

250 mm Length – Maximum Resolution for Complex Separations

The 250 mm length delivers the highest resolving power available in this format, ideal for the most challenging analytical separations.

• Maximum resolution compared to shorter column lengths

• Superior separation of closely related or co‑eluting analytes

• Highest peak capacity for complex matrices

✅ Recommended for method development, impurity profiling, and highly complex separations

3.0 mm ID – Solvent Saver for Analytical HPLC

The 3.0 mm internal diameter provides a balance between traditional HPLC and UHPLC formats.

• Similar run times to 4.6 mm ID columns

• Significantly reduced solvent consumption

• Lower flow rates with standard HPLC compatibility

✅ Ideal for labs seeking high resolution with reduced solvent cost and no UHPLC requirement

Flow Rate & System Requirements (3.0 mm ID, 4 µm)

✅ Compatible with standard HPLC systems

• Typical flow rate range: ~0.4 – 0.8 mL/min

• Moderate backpressure despite longer length

• Compatible with UV and MS detection systems

✅ Best Practice: Use guard columns and proper sample preparation to maximize performance and column life

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves co‑eluting and structurally similar compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Retention can be tuned via pH and ionic strength

✅ Ideal when traditional reversed phase or ion exchange phases cannot achieve sufficient separation

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under low pH and harsh conditions

• Resistant to hydrolysis

Faster Equilibration

• Improves throughput and reduces cycle times

No Strict Moisture Control (HILIC)

• Simplifies mobile phase preparation

Extended Column Lifetimes

• Durable for routine and demanding workflows

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Maximum Resolution Capability

• Ideal for complex and challenging separations

Solvent Reduction (3.0 mm ID)

• Lower operating costs vs standard 4.6 mm columns

Standard HPLC Compatibility (4 µm)

• No need for UHPLC system

Typical Applications

• Complex matrix separations

• Impurity profiling and method development

• Basic and positively charged compounds

• Polar and ionizable analytes

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Compatible with standard HPLC systems

• Designed for solvent‑efficient workflows

• pH control is critical for WCX selectivity

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

Please contact Customer Service for formal quotation, lead time, and ordering assistance.