Catalog Number - 40031-P21-100

40031-P21-100 Cogent UDA™ (weak cation exchange, WCX) Preparative HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering a uniquely powerful mixed‑mode separation mechanism that combines reversed phase, weak cation exchange, and HILIC retention in one stationary phase.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling multiple interaction modes:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This multi‑mode selectivity allows highly effective purification of polar, basic, and ionizable compounds, especially in complex matrices where traditional preparative C18 or ion exchange columns lack selectivity.

The TYPE‑C silica hydride base provides critical advantages:

• Faster equilibration between runs

• Reduced sensitivity to mobile phase moisture (ideal for HILIC methods)

• Extremely robust column lifetimes for repeated preparative use

• Minimal salt requirements in HILIC mode (cleaner fractions and easier downstream processing)

100 mm Length – Balanced Resolution for Preparative Workflows

The 100 mm length provides an ideal balance between throughput and resolving power for preparative separations.

• Higher resolution than 50 mm preparative columns

• Faster cycle times than longer 150 mm or 250 mm formats

• Increased peak capacity for moderately complex mixtures

✅ Recommended for routine preparative purification, process development, and scale‑up workflows

Flow Rate & System Requirements (21.2 mm ID, 4 µm)

⚠️ Requires preparative or process‑scale HPLC system capability

• Typical flow rate range: ~15 – 40 mL/min

• Moderate backpressure for preparative dimensions

• Designed for high‑flow, large‑volume injection workflows

• Compatible with large‑scale fraction collection systems

✅ Important: A specialized preparative HPLC system is required for columns of this size

✅ Best Practice: Use proper sample preparation and a matched guard column to protect the column and maintain performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Purification

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Retention tunable via pH and ionic strength

✅ Ideal when standard preparative C18 or ion exchange columns cannot achieve required purity

TYPE‑C Silica Advantages

Faster Equilibration

• Improves throughput and reduces downtime

No Strict Moisture Control (HILIC)

• Simplifies mobile phase preparation

Robust Column Lifetimes

• Designed for repeated purification cycles

Low Salt Elution (HILIC Mode)

• Cleaner fractions and improved downstream processing

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Preparative Scale (21.2 mm ID)

• Suitable for purification and scale‑up

Balanced Performance (100 mm Length)

• Strong resolution with efficient throughput

Typical Applications

• Preparative HPLC purification

• Process scale‑up from analytical methods

• Complex matrix separations

• Basic and ionizable compound purification

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires preparative or process‑scale HPLC system capability

• Designed for high‑flow operation (21.2 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.