Catalog Number - 40031-P21-150

40031-P21-150 Cogent UDA™ (weak cation exchange, WCX) Preparative HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering a highly specialized mixed‑mode separation mechanism that combines:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling powerful retention and selectivity for polar, basic, and ionizable compounds.

This multi‑mode functionality is particularly valuable in preparative purification of complex matrices, where conventional C18 or single‑mode ion exchange columns often lack sufficient selectivity.

The TYPE‑C silica hydride base provides important performance advantages:

• Faster equilibration between runs

• Reduced sensitivity to mobile phase moisture (ideal for HILIC methods)

• Extremely robust column lifetimes under repeated preparative use

• Minimal salt requirements in HILIC mode for cleaner fractions

150 mm Length – Enhanced Resolution for Preparative Separation

The 150 mm length provides increased resolving power for difficult separations at preparative scale.

• Higher resolution than 50 mm and 100 mm preparative columns

• Improved separation of closely related or co‑eluting compounds

• Increased peak capacity for complex sample mixtures

✅ Recommended for purification workflows requiring higher resolution and improved separation performance

Flow Rate & System Requirements (21.2 mm ID, 4 µm)

⚠️ Requires preparative or process‑scale HPLC system capability

• Typical flow rate range: ~15 – 40 mL/min

• Moderate pressure for 21.2 mm preparative column

• Designed for high‑flow, large‑volume injection workflows

• Compatible with large‑scale fraction collection systems

✅ Important: A specialized preparative HPLC system is required for columns of this size

✅ Best Practice: Use appropriate sample preparation and guard column protection to maintain performance and extend column lifetime

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Purification

• Resolves co‑eluting and structurally similar compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX functionality active at pH ≥ 6

• Retention can be tuned via pH and ionic strength

✅ Ideal when standard preparative C18 or ion exchange columns cannot achieve required purity

TYPE‑C Silica Advantages

Faster Equilibration

• Improves throughput in purification workflows

No Strict Moisture Control (HILIC)

• Simplifies method development and solvent handling

Robust Column Lifetimes

• Designed for repeated large‑scale use

Low Salt Elution (HILIC Mode)

• Cleaner fractions and simplified downstream processing

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Preparative Scale (21.2 mm ID)

• Suitable for large‑scale purification and scale‑up

Enhanced Resolution (150 mm Length)

• Ideal for complex and difficult separations

Typical Applications

• Preparative HPLC purification

• Process scale‑up

• Complex matrix separations

• Basic and ionizable compound purification

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires preparative or process‑scale HPLC system capability

• Designed for high‑flow operation (21.2 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.