Catalog Number - 40031-P21-250

40031-P21-250 Cogent UDA™ (weak cation exchange, WCX) Preparative HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering an exceptionally powerful mixed‑mode separation mechanism that combines:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

The UDA™ ligand (Undecanoic Acid), featuring an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enables highly selective retention of basic, polar, and ionizable compounds.

This multi‑mode capability provides a unique advantage for preparative purification of complex mixtures, where traditional C18 or single‑mode ion exchange columns often lack sufficient selectivity.

The TYPE‑C silica hydride base offers key performance advantages:

• Faster equilibration between preparative runs

• Reduced sensitivity to mobile phase moisture

• Extremely robust column lifetimes under repeated use

• Minimal salt requirements in HILIC mode (cleaner fractions and improved downstream processing)

250 mm Length – Maximum Resolution for Preparative Purification

The 250 mm length provides the highest resolving power in the preparative UDA™ column line.

• Maximum resolution compared to 50 mm, 100 mm, and 150 mm columns

• Superior separation of closely related and co‑eluting compounds

• Highest peak capacity for complex purification challenges

✅ Recommended for impurity isolation, final purification, and high‑purity production workflows

Flow Rate & System Requirements (21.2 mm ID, 4 µm)

⚠️ Requires preparative or process‑scale HPLC system capability

• Typical flow rate range: ~15 – 40 mL/min

• Increased backpressure due to longer column length

• Designed for high‑flow, high‑capacity purification

• Compatible with large‑scale fraction collection systems

✅ Important: A specialized preparative HPLC system is required for columns of this size

✅ Best Practice: Use proper sample preparation and a matched guard column to maintain column performance and longevity

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Purifications

• Resolves co‑eluting and structurally similar compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Selectivity tunable via pH and ionic strength

✅ Ideal when traditional preparative C18 or ion exchange columns are not sufficient

TYPE‑C Silica Advantages

Faster Equilibration

• Improves throughput in preparative workflows

No Strict Moisture Control (HILIC)

• Simplifies solvent preparation

Robust Column Lifetimes

• Designed for repeated large‑scale purification

Low Salt Elution (HILIC Mode)

• Cleaner fractions and simplified downstream processing

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Preparative Scale (21.2 mm ID)

• Ideal for large‑scale purification and scale‑up

Maximum Resolution Capability

• Best suited for difficult and complex separations

Typical Applications

• Preparative HPLC purification

• Impurity isolation and final product purification

• Complex matrix separations

• Basic and ionizable compound purification

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires preparative or process‑scale HPLC system capability

• Designed for high‑flow operation (21.2 mm ID columns)

• Increased system pressure due to 250 mm length

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.