Catalog Number - 40031-P21-50

40031-P21-50 Cogent UDA™ (weak cation exchange, WCX) Preparative HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), providing a uniquely powerful separation mechanism combining reversed phase, weak cation exchange, and HILIC retention in one stationary phase.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling multiple interaction modes:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This mixed‑mode selectivity is especially valuable for large‑scale purification of polar, ionizable, and structurally similar compounds, particularly in complex matrices where traditional prep columns lack selectivity.

The TYPE‑C silica hydride base provides significant advantages:

• Faster equilibration between preparative runs

• Reduced sensitivity to mobile phase moisture

• Extremely robust column lifetimes under repeated use

• Minimal salt requirements in HILIC mode (cleaner fractions and easier downstream processing)

50 mm Length – Fast Preparative Purification

The 50 mm length is optimized for speed and throughput in preparative workflows.

• Very fast purification cycles

• Reduced solvent consumption compared to longer columns

• Ideal for method scouting and early-stage scale‑up

✅ Recommended for high‑throughput preparative purification and rapid scale‑up workflows

Flow Rate & System Requirements (21.2 mm ID, 4 µm)

⚠️ Requires preparative or purification-scale HPLC system capability

• Typical flow rate range: ~15 – 40 mL/min

• Moderate backpressure for preparative column

• Designed for high‑flow, large‑volume injection workflows

• Compatible with large‑scale fraction collection systems

✅ Important: A specialized preparative HPLC system is required for columns of this size

✅ Best Practice: Use appropriate sample preparation and a matched guard column to protect the column and maintain performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Purifications

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Driven Selectivity Control

• WCX mechanism active at pH ≥ 6

• Retention tunable via pH and ionic strength

✅ Ideal when traditional preparative C18 or ion exchange columns cannot achieve required purity

TYPE‑C Silica Advantages

Faster Equilibration

• Improves throughput and reduces downtime

No Strict Moisture Control (HILIC)

• Simplifies mobile phase handling

Robust Column Lifetimes

• Designed for repeated preparative use

Low Salt Elution (HILIC Mode)

• Cleaner fractions and easier downstream processing

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

Preparative Scale (21.2 mm ID)

• Suitable for purification and scale‑up

Fast Throughput Design (50 mm Length)

• Ideal for rapid production workflows

Typical Applications

• Preparative HPLC purification

• Process scale‑up from analytical methods

• Complex matrix separations

• Basic and ionizable compound purification

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires preparative or process‑scale HPLC system capability

• Designed for high‑flow operation (21.2 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.