Catalog Number - 40031-P30-100

40031-P30-100 Cogent UDA™ (weak cation exchange, WCX) Preparative HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering a uniquely powerful mixed‑mode separation mechanism that combines:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling highly selective retention of polar, basic, and ionizable analytes.

This multi‑mode capability is especially valuable in large‑scale purification workflows, where traditional preparative columns may not provide sufficient selectivity for complex sample matrices.

The TYPE‑C silica hydride base provides key performance advantages:

• Faster equilibration between preparative runs

• Reduced sensitivity to mobile phase moisture (ideal for HILIC methods)

• Extremely robust column lifetimes under repeated use

• Minimal salt requirements in HILIC mode (cleaner fractions and easier downstream processing)

100 mm Length – Balanced Performance for Preparative Workflows

The 100 mm length provides an optimal balance between throughput and resolving power in large‑scale purification.

• Higher resolution than 50 mm columns

• Faster run times than 150 mm and 250 mm formats

• Increased peak capacity for moderately complex mixtures

✅ Recommended for routine preparative purification, process development, and scale‑up workflows

Flow Rate & System Requirements (30 mm ID, 4 µm)

⚠️ Requires preparative or process‑scale HPLC system capability

• Typical flow rate range: ~25 – 60 mL/min

• Designed for very high‑flow operation

• Moderate backpressure for 30 mm ID columns

• Compatible with large‑scale fraction collection systems

✅ Important: A specialized preparative HPLC system is required for columns of this size

✅ Best Practice: Use proper sample preparation and a matched guard column to protect the column and maintain long‑term performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Purification

• Resolves co‑eluting and structurally similar compounds

• Highly effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Retention tunable via pH and ionic strength

✅ Ideal when standard preparative C18 or ion exchange columns cannot achieve sufficient purity

TYPE‑C Silica Advantages

Faster Equilibration

• Improves throughput and reduces downtime

No Strict Moisture Control (HILIC)

• Simplifies solvent preparation

Robust Column Lifetimes

• Designed for repeated large‑scale purification

Low Salt Elution (HILIC Mode)

• Cleaner fractions and improved downstream processing

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in one column

High‑Capacity Prep Scale (30 mm ID)

• Ideal for large‑volume purification and production

Balanced Performance (100 mm Length)

• Strong resolution with efficient throughput

Typical Applications

• Preparative HPLC purification

• Production‑scale separations

• Complex matrix purification

• Basic and ionizable compound isolation

• Pharmaceutical and research workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires preparative or process‑scale HPLC system capability

• Designed for very high‑flow operation (30 mm ID columns)

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.