Catalog Number - 40031-SP250

40031-SP250 Cogent UDA™ (weak cation exchange, WCX) Semi‑Prep HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), providing a uniquely powerful mixed‑mode separation mechanism that combines:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling highly selective retention of basic, polar, and ionizable analytes.

This multi‑mode capability is especially valuable in semi‑preparative purification workflows, where difficult separations in complex matrices require more than traditional C18 or ion exchange selectivity.

The TYPE‑C silica hydride base provides critical advantages:

• Faster equilibration between runs

• Reduced sensitivity to mobile phase moisture (ideal for HILIC methods)

• Extremely robust column lifetimes

• Minimal salt requirements in HILIC mode (cleaner fractions and better MS compatibility)

250 mm Length – Maximum Resolution for Semi‑Prep Purification

The 250 mm length delivers the highest resolving power in semi‑prep format, ideal for the most challenging purification tasks.

• Maximum resolution compared to shorter semi‑prep columns

• Superior separation of closely related or co‑eluting compounds

• Highest peak capacity for complex mixtures

✅ Recommended for impurity isolation, scale‑up purification, and high‑purity production workflows

Flow Rate & System Requirements (10 mm ID, 4 µm)

⚠️ Requires semi‑preparative HPLC system capability

• Typical flow rate range: ~4 – 12 mL/min

• Increased backpressure due to 250 mm length, but manageable

• Designed for high‑flow purification workflows

• Compatible with fraction collectors and preparative detectors

✅ Important: A specialized HPLC system is required for columns of this size

✅ Best Practice: Use proper sample preparation and a matched guard column to protect the column and maintain performance

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Purifications

• Resolves co‑eluting and structurally similar compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Selectivity tunable via pH and ionic strength

✅ Ideal when traditional prep C18 or ion exchange columns cannot achieve required separation or purity

TYPE‑C Silica Advantages

Faster Equilibration

• Improves throughput and reduces downtime

No Strict Moisture Control (HILIC)

• Simplifies mobile phase handling

Robust Column Lifetimes

• Designed for repeated semi‑prep use

Low Salt Elution (HILIC Mode)

• Cleaner fractions and improved downstream processing

Cogent UDA™ Column Advantages

Mixed‑Mode Flexibility

• RP, HILIC, and WCX in one column

Maximum Resolution Capability

• Ideal for difficult separations and purification challenges

Semi‑Prep Scale (10 mm ID)

• Supports purification and analytical scale‑up

Balanced Performance (4 µm Particles)

• Excellent efficiency with manageable pressure

Typical Applications

• Semi‑preparative HPLC purification

• Scale‑up from analytical LC methods

• Complex matrix separations

• Impurity isolation and purification

• Basic and ionizable compound purification

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires semi‑preparative HPLC system capability

• Designed for high‑flow operation (10 mm ID columns)

• Increased pressure due to longer column length

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

Please contact Customer Service for formal quotation, lead time, and ordering assistance.