Catalog Number - 40036-05P-2

The 40036‑05P‑2 Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase provides strong, reproducible interaction with polar analytes and amine‑containing compounds, while the silica‑hydride surface minimizes water‑layer instability commonly observed with conventional bonded phases.

This column is optimized for HILIC methods requiring reliable retention and rapid equilibration, particularly for highly polar analytes.

Application Focus: Amide Chemistry for HILIC Separations

Amide stationary phases are specifically selected for the analysis of highly polar compounds, especially those containing amine or hydroxyl functionality. The Amide phase is particularly effective for:

• Sugars and sugar alcohols

• Amino‑containing analytes

• Polar metabolites

• Other HILIC‑appropriate polar compounds

The TYPE‑C™ silica hydride backbone supports stable retention and improved reproducibility compared to traditional silica‑based amide phases.

Key Advantages of Cogent TYPE‑C™ Amide Technology

Compared to conventional amide HILIC columns, this column offers:

• Faster equilibration between injections

• Improved retention stability over wide mobile‑phase compositions

• Robust column lifetime under high‑organic HILIC conditions

• Reduced water‑layer variability due to silica‑hydride surface chemistry

These characteristics make the Amide column a dependable choice for routine and method‑development HILIC workflows.

Why Choose the 2.1 mm × 50 mm Format

The 2.1 mm ID × 50 mm length configuration is designed for speed, efficiency, and LC‑MS compatibility:

• Short run times and high throughput

• Rapid re‑equilibration between injections

• Reduced solvent consumption

• Excellent compatibility with LC‑MS and LC‑MS/MS detection

This narrow‑bore format requires an HPLC system capable of precise low‑flow delivery and appropriately sized flow cells, making it a popular choice for modern LC‑MS workflows.

Particle Size and Performance

The 4 µm particle size offers a practical balance between efficiency, backpressure, and robustness, delivering reliable peak shape and resolution on standard analytical and LC‑MS‑capable HPLC systems.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Optimized flow rates should consider mobile‑phase viscosity, column temperature, and system pressure limits.