CUSTOM ITEM: HPLC Column, Amide, 4um, 3.0mm ID x 50mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑05P‑3 Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes water‑layer instability commonly associated with conventional bonded phases.
This column is produced as a custom, made‑to‑order item and is optimized for laboratories seeking solvent efficiency and analytical flexibility without sacrificing resolution.
Application Focus: Amide Chemistry for HILIC Separations
Amide stationary phases are particularly suited for highly polar analytes that require stable HILIC retention. The Amide phase is commonly selected for:
Sugars and sugar alcohols
Amine‑containing compounds
Polar metabolites
Other analytes requiring strong HILIC interaction
The TYPE‑C™ silica hydride backbone supports stable retention and improved reproducibility compared to traditional amide‑bonded silica phases.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide HILIC columns, this column provides:
Faster equilibration between injections
Improved retention stability over varying mobile‑phase compositions
Robust column lifetime under high‑organic HILIC conditions
Reduced moisture sensitivity, improving run‑to‑run consistency
These attributes make the Amide TYPE‑C™ column well suited for both routine analysis and method development.
Why Choose the 3.0 mm × 50 mm Format
The 3.0 mm ID × 50 mm length configuration offers a balance between speed, solvent efficiency, and system compatibility:
Run times similar to 4.6 mm ID columns
Lower operating flow rates, reducing mobile‑phase consumption
Lower backpressure than smaller‑ID formats
Compatible with standard analytical HPLC systems, with no specialized low‑flow hardware required
This format is ideal when laboratories want solvent savings without transitioning to narrow‑bore or LC‑MS‑specific configurations.
Particle Size and Performance
The 4 µm particle size delivers reliable efficiency and peak shape while maintaining manageable backpressure, providing dependable performance on standard analytical HPLC platforms.
System Requirements and Flow Rates
Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable