HPLC Column, Amide, 4um, 4.6mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑10P Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase delivers strong, consistent interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface improves retention stability relative to conventional amide‑bonded silica phases.
This column is optimized for laboratories requiring greater resolving power than 50 mm formats, while maintaining efficient run times on standard analytical HPLC systems.
Application Focus: Amide Chemistry for HILIC Separations
Amide stationary phases are selected for the analysis of highly polar analytes that require reliable HILIC retention. The Amide phase is particularly effective for:
Sugars and sugar alcohols
Amine‑containing compounds
Polar metabolites
Other HILIC‑appropriate polar analytes
The TYPE‑C™ silica hydride backbone supports stable retention and improved reproducibility, minimizing variability associated with moisture‑sensitive silica surfaces.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to traditional amide HILIC columns, this column provides:
Faster equilibration between injections
Improved retention stability across mobile‑phase compositions
Robust column lifetime under high‑organic HILIC conditions
Reduced water‑layer variability, improving run‑to‑run consistency
These characteristics make the Amide TYPE‑C™ column suitable for both routine analysis and method development.
Why Choose the 4.6 mm × 100 mm Format
The 4.6 mm ID × 100 mm length configuration is often selected when shorter run times are needed, but 50 mm columns do not provide sufficient resolution:
Greater resolving power than 50 mm formats
Efficient run times relative to longer analytical columns
Standard analytical flow rates compatible with most HPLC systems
Well suited for high‑throughput analytical workflows requiring added separation confidence
This format offers a practical balance between speed, robustness, and resolution.
Particle Size and Performance
The 4 µm particle size delivers reliable efficiency and peak shape with manageable backpressure, supporting consistent performance on standard analytical HPLC instrumentation.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.