HPLC Column, Amide, 4um, 2.1mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑10P‑2 Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase provides strong interaction with amine‑containing and highly polar analytes, while the silica‑hydride backbone stabilizes retention behavior and minimizes moisture‑related variability common to traditional silica‑based HILIC columns.
This column is optimized for high‑resolution analytical and LC‑MS workflows, where additional separation power is required beyond shorter column formats.
Application Focus: Amide Chemistry for HILIC Separations
Amide stationary phases are selected for the analysis of highly polar analytes requiring strong and reproducible HILIC retention. The Amide phase is particularly effective for:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds
The TYPE‑C™ silica hydride surface supports consistent retention and improved reproducibility compared to conventional amide‑bonded silica phases, especially under high‑organic mobile‑phase conditions.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to traditional amide HILIC columns, this column provides:
Faster equilibration between gradient runs
Improved retention stability across varying mobile‑phase compositions
Robust column lifetime under demanding HILIC conditions
Reduced water‑layer variability, improving run‑to‑run consistency
These attributes make the Amide TYPE‑C™ column well suited forboth routine analysis and HILIC method development.
Why Choose the 2.1 mm × 100 mm Format
The 2.1 mm ID × 100 mm length configuration is one of the most popular Amide column formats when increased resolution is desired, particularly for LC‑MS applications:
Higher resolving power than 50 mm formats
Improved peak capacity for complex polar mixtures
Reduced solvent consumption compared to larger‑diameter columns
Excellent compatibility with LC‑MS and LC‑MS/MS detection
This narrow‑bore format requires an HPLC system capable of accurate and reliable low‑flow delivery, making it ideal for modern mass‑spectrometry‑based workflows.
Particle Size and Performance
The 4 µm particle size delivers a balance of efficiency, robustness, and manageable backpressure, producing reliable peak shapes and reproducible performance on analytical and LC‑MS‑capable HPLC systems.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
System capability: Requires stable, accurate low‑flow performance
Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates
Flow rates should be optimized considering mobile‑phase viscosity, column temperature, and system pressure limits.