CUSTOM ITEM: HPLC Column, Amide, 4um, 3.0mm ID x 100mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑10P‑3 Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface mitigates the moisture‑layer instability often encountered with conventional bonded‑silica amide columns.
This column is produced as a custom, made‑to‑order item and is optimized for laboratories seeking solvent efficiency and broad system compatibility without compromising chromatographic performance.
Application Focus: Amide Chemistry for HILIC Separations
Amide stationary phases are particularly well suited for highly polar analytes requiring stable HILIC retention. The Amide phase is commonly selected for:
Sugars and sugar alcohols
Amine‑containing compounds
Polar metabolites
Other HILIC‑appropriate polar analytes
The TYPE‑C™ silica hydride backbone supports consistent retention and improved reproducibility compared to traditional amide‑bonded silica phases, especially under high‑organic mobile‑phase conditions.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide HILIC columns, this column offers:
Faster equilibration between injections
Improved retention stability across mobile‑phase compositions
Robust column lifetime under demanding HILIC conditions
Reduced moisture sensitivity, improving run‑to‑run reproducibility
These characteristics make the Amide TYPE‑C™ column effective for both routine analysis and method development.
Why Choose the 3.0 mm × 100 mm Format
The 3.0 mm ID × 100 mm length configuration provides an efficient balance between resolution, solvent savings, and system compatibility:
Run times comparable to 4.6 mm ID columns
Lower operating flow rates, reducing solvent consumption
Lower backpressure than narrow‑bore formats
Compatible with standard analytical HPLC systems, with no specialized low‑flow hardware required
This format is ideal when increased resolution is desired without transitioning to narrow‑bore or LC‑MS‑only configurations.
Particle Size and Performance
The 4 µm particle size delivers reliable efficiency and peak shape while maintaining manageable backpressure, providing dependable performance on standard analytical HPLC platforms.
System Requirements and Flow Rates
Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable